Application

ELISA: A concentration of at least 0.5µg/mL (100µL/well) of AB2148P is required to detect 0.2-0.4 ng/well of recombinant mTNF-alpha.

Western Blot: A concentration of 0.1-0.2 µg/mL of AB2148P is required to detect 1.5-3.0 ng/lane of mTNF-alpha, under either reducing or non-reducing conditions.

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mTNF-a (0.50 ng/ml), a concentration of 0.010-0.015 µg/ml of AB2148P is required.

Optimal working dilutions must be determined by the end user

Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody validated for use in ELISA, NEUT & WB.

Immunogen

Highly pure (>98%) recombinant Mouse TNF-alpha.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB1450P

Physical form

Immunoglobulin purified by affinity chromatography employing immobilized mTNF-alpha matrix. A sterile filtered antibody solution (1mg/mL) was lyophilized from 0.5X PBS, pH 7.2. Reconstitute with sterile distilled water.

Specificity

Reacts with Mouse Tumor Necrosis Factor-alpha (TNF-alpha), a potent immuno-modulator that mediates a wide range of immune and inflammatory responses including the activation of B and T cells.